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horseradish peroxidase hrp conjugated donkey polyclonal anti sheep igg  (R&D Systems)


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    R&D Systems horseradish peroxidase hrp conjugated donkey polyclonal anti sheep igg
    Horseradish Peroxidase Hrp Conjugated Donkey Polyclonal Anti Sheep Igg, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sheep+igg+hrp/Sheep+IgG+Horseradish+Peroxidase-conjugated+Antibody/pmc12970870-42-3-13
    Average 95 stars, based on 205 article reviews
    horseradish peroxidase hrp conjugated donkey polyclonal anti sheep igg - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Teneurin-4, a transmembrane protein, is a novel regulator that suppresses chondrogenic differentiation.
    Article Snippet: After that, the samples were blocked with Power Block Universal Blocking Reagent (BioGenex Laboratories, Fremont, CA). .. Proteins were detected by primary and secondary antibodies, as follows: Ten-4 (R&D systems), type II collagen (Daiichi Fine Chemical, Toyama, Japan), sheep IgG-HRP (R&D Systems), biotinylated mouse IgG (Vector Laboratories, Burlingame, CA), and DAB staining kit (Thermo Scientific, Rockford, IL) or VECTASTAIN ABC reagent (Vector Laboratories). .. The mouse chondrogenic cell line ATDC5 (RIKEN BRC, Tsukuba, Japan) was cultured in DMEM containing 5% FBS (GE Healthcare, Little Chalfont, UK), 100units/ml penicillin, and 100mg/ml streptomycin (Life Technologies) (Proliferation medium) under 5% CO2.

    Western Blot:

    Article Title: Teneurin-4, a transmembrane protein, is a novel regulator that suppresses chondrogenic differentiation.
    Article Snippet: .. Protein samples from ATDC5 cells were analyzed by Western blotting as described previously.10 The following antibodies were used to detect proteins: Ten-4 (R&D Systems), atubulin (Sigma–Aldrich), phospho-ERK1/2 (Thr202/Tyr204), ERK1/2 (Cell Signaling Technology, Danvers, MA), sheep IgG-HRP (R&D Systems), rabbit IgG-HRP (Sigma-Aldrich), and mouse IgG-HRP (GE Healthcare). ..



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    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Journal: Frontiers in Veterinary Science

    Article Title: Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA

    doi: 10.3389/fvets.2026.1775573

    Figure Lengend Snippet: Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Article Snippet: Optimization parameters included: (i) coating concentration of the single antigen or antigen mixture, (ii) serum dilution, and (iii) working dilution of the HRP-conjugated secondary antibody (Rabbit anti-sheep IgG (H/L): HRP; BIO-RAD, 5184–2,504).

    Techniques: Recombinant, Titration, Concentration Assay